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Title: Hairpin-structured probe conjugated nano-graphene oxide for the cellular detection of connective tissue growth factor mRNA
Authors: Wang, Min
Wiraja, Christian
Wee, Meiling
Hu, Lianzhe
Xu, Chenjie
Yeo, David Chen Loong
Keywords: Engineering::Chemical engineering
Abnormal Scar
Issue Date: 2018
Source: Wang, M., Wiraja, C., Wee, M., Yeo, D. C. L., Hu, L., & Xu, C. (2018). Hairpin-structured probe conjugated nano-graphene oxide for the cellular detection of connective tissue growth factor mRNA. Analytica Chimica Acta, 1038, 140-147. doi:10.1016/j.aca.2018.07.016
Series/Report no.: Analytica Chimica Acta
Abstract: Identification of abnormal scars at their early stage has attracted increasing attentions as the scars can only be assessed qualitatively and subjectively upon maturity, when no invasive procedure is involved. This report introduces a fluorescent probe that targets a potential abnormal scar biomarker (connective tissue growth factor (CTGF) mRNA) in skin fibroblasts. This probe is constructed of hairpin-structured probes (HPs) targeting CTGF mRNA and the nano-graphene oxide (nano-GO) base. The HPs are non-covalently absorbed on the surface of nano-GO, which pre-quenches the fluorescence of HPs. Close proximity of complementary CTGF mRNA would lead to preferential HP hybridization and dissociation from nano-GO, which restores the fluorescence signal from HPs. Utilizing this probe, we can distinguish abnormal fibroblasts derived from abnormal scars and assess the effectiveness of anti-scarring drugs like Repsox and transforming growth factor-beta type I receptor (TGF-βRI) siRNA.
ISSN: 0003-2670
DOI: 10.1016/j.aca.2018.07.016
Schools: School of Chemical and Biomedical Engineering 
School of Biological Sciences 
Rights: © 2018 Elsevier B.V. All rights reserved. This paper was published in Analytica Chimica Acta and is made available with permission of Elsevier B.V.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
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