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Title: MINDY1 is a downstream target of the polyamines and promotes embryonic stem cell self‐renewal
Authors: Nazreen Abdul Muthalif
Vardy, Leah Anne
James, Christina
Zhao, Tian Yun
Rahim, Anisa
Saxena, Parul
Uemura, Takeshi
Tsuneyoshi, Norihiro
Ong, Sheena
Igarashi, Kazuei
Lim, Chin Yan
Dunn, Norris Ray
Keywords: Polyamines
Embryonic Stem Cells
Science::Biological sciences
Issue Date: 2018
Source: James, C., Zhao, T. Y., Rahim, A., Saxena, P., Nazreen Abdul Muthalif., Uemura, T., . . . Vardy, L. A. (2018). MINDY1 is a downstream target of the polyamines and promotes embryonic stem cell self‐renewal. STEM CELLS, 36(8), 1170-1178. doi:10.1002/stem.2830
Series/Report no.: STEM CELLS
Abstract: Embryonic stem cells have the ability to self‐renew or differentiate and these processes are under tight control. We previously reported that the polyamine regulator AMD1 is critical for embryonic stem cell self‐renewal. The polyamines putrescine, spermidine, and spermine are essential organic cations that play a role in a wide array of cellular processes. Here, we explore the essential role of the polyamines in the promotion of self‐renewal and identify a new stem cell regulator that acts downstream of the polyamines: MINDY1. MINDY1 protein levels are high in embryonic stem cells (ESCs) and are dependent on high polyamine levels. Overexpression of MINDY1 can promote ESC self‐renewal in the absence of the usually essential cytokine Leukemia Inhibitory Factor (LIF). MINDY1 protein is prenylated and this modification is required for its ability to promote self‐renewal. We go on to show that Mindy1 RNA is targeted for repression by mir‐710 during Neural Precursor cell differentiation. Taken together, these data demonstrate that high polyamine levels are required for ESC self‐renewal and that they function, in part, through promotion of high MINDY1 levels.
ISSN: 1066-5099
DOI: 10.1002/stem.2830
Rights: © 2018 The Author(s) published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. This is an open access article under the terms of the Creative Commons AttributionNonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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