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Title: Biomimicking fiber scaffold as an effective in vitro and in vivo microRNA screening platform for directing tissue regeneration
Authors: Zhang, Na
Milbreta, Ulla
Chin, Jiah Shin
Pinese, Coline
Lin, Junquan
Shirahama, Hitomi
Jiang, Wei
Liu, Hang
Mi, Ruifa
Hoke, Ahmet
Wu, Wutian
Chew, Sing Yian
Keywords: Electrospinning
DRNTU::Engineering::Chemical engineering
Contact Guidance
Issue Date: 2019
Source: Zhang, N., Milbreta, U., Chin, J. S., Pinese, C., Lin, J., Shirahama, H., . . . Chew, S. Y. (2019). Biomimicking fiber scaffold as an effective in vitro and in vivo microRNA screening platform for directing tissue regeneration. Advanced Science, 6(9), 1800808-. doi:10.1002/advs.201800808
Series/Report no.: Advanced Science
Abstract: MicroRNAs effectively modulate protein expression and cellular response. Unfortunately, the lack of robust nonviral delivery platforms has limited the therapeutic application of microRNAs. Additionally, there is a shortage of drug‐screening platforms that are directly translatable from in vitro to in vivo. Here, a fiber substrate that provides nonviral delivery of microRNAs for in vitro and in vivo microRNA screening is introduced. As a proof of concept, difficult‐to‐transfect primary neurons are targeted and the efficacy of this system is evaluated in a rat spinal cord injury model. With this platform, enhanced gene‐silencing is achieved in neurons as compared to conventional bolus delivery (p < 0.05). Thereafter, four well‐recognized microRNAs (miR‐21, miR‐222, miR‐132, and miR‐431) and their cocktails are screened systematically. Regardless of age and origin of the neurons, similar trends are observed. Next, this fiber substrate is translated into a 3D system for direct in vivo microRNA screening. Robust nerve ingrowth is observed as early as two weeks after scaffold implantation. Nerve regeneration in response to the microRNA cocktails is similar to in vitro experiments. Altogether, the potential of the fiber platform is demonstrated in providing effective microRNA screening and direct translation into in vivo applications.
DOI: 10.1002/advs.201800808
Rights: © 2019 Nanyang Technological University. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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