Dynamic imaging of micro-particles in 3D using lensless in-line digital holographic microscopy

Abstract

In this paper, dynamic imaging of micro-particles in 3D using lensless in-line digital holographic microscopy (LIDHM) is explored. The methodology presented retains the reconstructed pixels size used for the particles analysis at different depth locations under converging wave reconstruction geometry. Experiments studying the 3D visualization of copolymer microsphere suspensions in distilled water are presented. The dynamic behavior of microspheres in 1mm cube volume is captured and the numerical reconstruction provides their volumetric flow behavior. From the plot the 3D visualization and the location of each particle, its depth and dynamic behavior can be observed clearly. The proposed work is useful for tracking the 3D dynamic behavior of particles and can be used to predict the motion of the moving particles in volume.