Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/94319
Title: Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy
Authors: Zhang, Enming
Xue, Renhao
Soo, Jianchow
Chen, Peng
Keywords: DRNTU::Science::Medicine::Biomedical engineering
Issue Date: 2008
Source: Zhang, E., Xue, R., Soo, J. & Chen, P. (2008). Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy. Pflügers Archiv European Journal of Physiology, 457, 211-222.
Series/Report no.: Pflügers archiv European journal of physiology
Abstract: Exocytosis of neurotransmitter or hormone-filled vesicles is a highly dynamic process regulated by various proteins and lipids. As mainly revealed indirectly by the electrophysiological methods, exocytosis is believed to involve multiple kinetic steps in which vesicles transit from one state to another. Using total internal reflection fluorescence microscopy which enables direct visualization of individual vesicles, we developed an analytical framework to track and analyze vesicle dynamics. We demonstrated that all subplasmalemmal vesicles generally undergo constant and caged Brownian motion. And they can be classified into three populations that differ in their motion characteristics and fusion competence. Furthermore, we showed that these vesicle pools are differentially modulated by phorbol-12-myristate-13-acetate, a phorbol ester analog to endogenous diacylglycerol, through both protein-kinase- C-dependent and -independent pathways.
URI: https://hdl.handle.net/10356/94319
http://hdl.handle.net/10220/7522
DOI: 10.1007/s00424-008-0485-6
Rights: © 2008 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Pflügers Archiv European Journal of Physiology, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1007/s00424-008-0485-6]
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

SCOPUSTM   
Citations 20

12
Updated on Jan 28, 2023

Web of ScienceTM
Citations 20

13
Updated on Feb 2, 2023

Page view(s) 5

924
Updated on Feb 4, 2023

Download(s) 10

372
Updated on Feb 4, 2023

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.