Graphene-based biosensors for detection of bacteria and their metabolic activities

Abstract

Graphene, which is a recently discovered single-atom-thick planar sheet of carbon atoms perfectly

arranged in a honeycomb lattice, has great potential in biosensing owing to its extraordinary electrical,

physical, and optical properties. In this work, we demonstrate a graphene based biosensor to

electrically detect E. coli bacteria with high sensitivity and specificity. The large-sized graphene film was

grown by chemical vapor deposition and functionalized with anti-E. coli antibodies and passivation

layer. Significant conductance increase of the graphene device was observed after exposure to E. coli

bacteria at a concentration as low as 10 cfu/mL, while no significant response was triggered by high

concentration of the another bacterial strain. In addition, this biosensor was employed to detect the

glucose induced metabolic activities of the bound E. coli bacteria in real time. This simple, fast,

sensitive, and label-free nanoelectronic biosensor, in principle, could serve as a high throughput

platform for detection of any pathogenic bacteria, and for functional studies or screening of

antibacterial drugs.