Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/94545
Title: Rapid amplification of genetically modified organisms using a circular ferrofluid-driven PCR microchip
Authors: Sun, Yi
Kwok, Yien Chian
Lee, Peter Peng Foo
Nguyen, Nam-Trung
Keywords: DRNTU::Engineering::Mechanical engineering
Issue Date: 2009
Source: Sun, Y., Kwok, Y. C., Lee, P. F. P., & Nguyen, N. T. (2009). Rapid amplification of genetically modified organisms using a circular ferrofluid-driven PCR microchip. Analytical and Bioanalytical Chemistry, 394(5), 1505-1508.
Series/Report no.: Analytical and bioanalytical chemistry
Abstract: The use of genetically modified organisms (GMOs) as food and in food products is becoming more and more widespread. Polymerase chain reaction (PCR) technology is extensively used for the detection of GMOs in food products in order to verify compliance with labeling requirements. In this paper, we present a novel close-loop ferrofluid-driven PCR microchip for rapid amplification of GMOs. The microchip was fabricated in polymethyl methacrylate by CO2 laser ablation and was integrated with three temperature zones. PCR solution was contained in a circular closed microchannel and was driven by magnetic force generated by an external magnet through a small oil-based ferrofluid plug. Successful amplification of genetically modified soya and maize were achieved in less than 13 min. This PCR microchip combines advantages of cycling flexibility and quick temperature transitions associated with two existing microchip PCR techniques, and it provides a cost saving and less time-consuming way to conduct preliminary screening of GMOs.
URI: https://hdl.handle.net/10356/94545
http://hdl.handle.net/10220/7753
DOI: 10.1007/s00216-009-2808-7
Rights: © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and Bioanalytical Chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: DOI: [http://dx.doi.org/10.1007/s00216-009-2808-7].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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