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https://hdl.handle.net/10356/94659
Title: | Enhanced electrophoretic DNA separation in photonic crystal fiber | Authors: | Sun, Yi Nguyen, Nam-Trung Kwok, Yien Chian |
Keywords: | DRNTU::Engineering::Mechanical engineering | Issue Date: | 2009 | Source: | Sun, Y., Nguyen, N. T., & Kwok, Y. C. (2009). Enhanced electrophoretic DNA separation in photonic crystal fiber. Analytical and Bioanalytical Chemistry, 394(6), 1707-1710. | Series/Report no.: | Analytical and bioanalytical chemistry | Abstract: | Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip. | URI: | https://hdl.handle.net/10356/94659 http://hdl.handle.net/10220/7752 |
DOI: | 10.1007/s00216-009-2833-6 | Rights: | © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | MAE Journal Articles |
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