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Title: Enhanced electrophoretic DNA separation in photonic crystal fiber
Authors: Sun, Yi
Nguyen, Nam-Trung
Kwok, Yien Chian
Keywords: DRNTU::Engineering::Mechanical engineering
Issue Date: 2009
Source: Sun, Y., Nguyen, N. T., & Kwok, Y. C. (2009). Enhanced electrophoretic DNA separation in photonic crystal fiber. Analytical and Bioanalytical Chemistry, 394(6), 1707-1710.
Series/Report no.: Analytical and bioanalytical chemistry
Abstract: Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip.
DOI: 10.1007/s00216-009-2833-6
Rights: © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at:
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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