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Title: Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells
Authors: Kang, Tae-Hong
Park, Do-Young
Choi, Yoon Ha
Kim, Kyung-Jin
Yoon, Ho Sup
Kim, Kyong-Tai
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2007
Source: Kang, T. H., Park, D. Y., Choi, Y. H., Kim, K. J., Yoon, H. S., & Kim, K. T. (2007). Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells. Molecular and Cellular Biology, 27(24), 8533-8546.
Series/Report no.: Molecular and cellular biology
Abstract: Mitotic chromatin condensation is essential for cell division in eukaryotes. Posttranslational modification of the N-terminal tail of histone proteins, particularly by phosphorylation by mitotic histone kinases, may facilitate this process. In mammals, aurora B is believed to be the mitotic histone H3 Ser10 kinase; however, it is not sufficient to phosphorylate H3 Ser10 with aurora B alone. We show that histone H3 is phosphorylated by vaccinia-related kinase 1 (VRK1). Direct phosphorylation of Thr3 and Ser10 in H3 by VRK1 both in vitro and in vivo was observed. Loss of VRK1 activity was associated with a marked decrease in H3 phosphorylation during mitosis. Phosphory¬lation of Ser10 by VRK1 is similar to that by aurora B. Moreover, expression and chromatin localization of VRK1 depended on the cell cycle phase. Overexpression of VRK1 resulted in a dramatic condensation of nuclei. Our findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals.
ISSN: 0270-7306
DOI: 10.1128/MCB.00018-07
Rights: © 2007 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Molecular and Cellular Biology, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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