Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLee, Ashlynn L. Z.en
dc.contributor.authorWang, Yongen
dc.contributor.authorYe, Wen-Huien
dc.contributor.authorYoon, Ho Supen
dc.contributor.authorChan, Sui Yungen
dc.contributor.authorYang, Yi-Yanen
dc.identifier.citationLee, A. L., Wang, Y., Ye, W. H., Yoon, H. S., Chan, S. Y., & Yang, Y. Y. (2008). Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles. Biomaterials, 29(9), 1224-1232.en
dc.description.abstractCationic core/shell nanoparticles self-assembled from biodegradable, cationic and amphiphilic copolymer poly{N-methyldietheneaminesebacate)-co-[(cholesteryloxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate}, P(MDS-co-CES), were fabricated and employed to deliver lectin A-chain, an anticancer glycoprotein. Lectin A-chain was efficiently bound onto the surfaces of the nanoparticles at high mass ratios of nanoparticles to lectin A-chain. The nanoparticle/lectin A-chain complexes had an average size of approximately 150 nm with zeta potential of about +30 mV at the mass ratio of 50 or above while the BioPorter/lectin A-chain complexes had a larger particle size and relatively lower zeta potential (150 nm vs. 455 nm; +30 mV vs. +20 mV). Therefore, the cellular uptake of nanoparticle/lectin A-chain com-plexes was much greater than that of BioPorter/lectin A-chain complexes. The results obtained from cytotoxicity tests show that lectin A-chain delivered by the nanoparticles was significantly more toxic against MDA-MB-231, HeLa, HepG2 and 4T1 cell lines when compared to Bio-Porter, and IC50 of lectin A-chain delivered by the nanoparticles was 0.2, 0.5, 10 and 50 mg/l, respectively, while that of lectin A-chain delivered by BioPorter was higher than 100 mg/l in all cell lines tested. These nano-sized particles may provide an efficient approach for intracellular delivery of biologically active proteins.en
dc.rights© 2007 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomaterials, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at:
dc.titleEfficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticlesen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionAccepted versionen
item.fulltextWith Fulltext-
Appears in Collections:SBS Journal Articles
Files in This Item:
File Description SizeFormat 
16.Efficient intracellular delivery of functional proteins using.pdf679.06 kBAdobe PDFThumbnail

Citations 5

Updated on Mar 21, 2023

Web of ScienceTM
Citations 5

Updated on Mar 27, 2023

Page view(s) 5

Updated on Mar 29, 2023

Download(s) 5

Updated on Mar 29, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.