Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/95360
Title: Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae
Authors: Basak, Sandip
Balakrishna, Asha Manikkoth
Manimekalai, Malathy Sony Subramanian
Grüber, Gerhard
Keywords: DRNTU::Science::Chemistry::Biochemistry
DRNTU::Science::Chemistry::Crystallography
Issue Date: 2012
Source: Basak, S., Balakrishna, A. M., Manimekalai, M. S. S., & Grüber, G. (2012). Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68(9), 1055-1059.
Series/Report no.: Acta crystallographica section F structural biology and crystallization communications
Abstract: V-ATPases are very complex multi-subunit enzymes which function as proton-pumping rotary nanomotors. The rotary and coupling subunit F (F1-94) was crystallized by the hanging-drop vapour-diffusion method. The native crystals diffracted to a resolution of 2.64 Å and belonged to space group C2221, with unit-cell parameters a = 47.21, b = 160.26, c = 102.49 Å. The selenomethionyl form of the F1-94 I69M mutant diffracted to a resolution of 2.3 Å and belonged to space group C2221, with unit-cell parameters a = 47.22, b = 160.83, c = 102.74 Å. Initial phasing and model building suggested the presence of four molecules in the asymmetric unit.
URI: https://hdl.handle.net/10356/95360
http://hdl.handle.net/10220/9180
ISSN: 1744-3091
DOI: 10.1107/S1744309112032526
Rights: © 2012 International Union of Crystallography. This paper was published in Acta Crystallographica Section F Structural Biology and Crystallization Communications and is made available as an electronic reprint (preprint) with permission of International Union of Crystallography. The paper can be found at the following official DOI: [http://dx.doi.org/10.1107/S1744309112032526]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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