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Title: Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
Authors: Rai, Varun
Hapuarachchi, Hapuarachchige C.
Ng, Lee Ching
Soh, Siew Hwa
Leo, Yee Sin
Toh, Chee-Seng
Issue Date: 2012
Source: Rai, V., Hapuarachchi, H. C., Ng, L. C., Soh, S. H., Leo, Y. S., & Toh, C. S. (2012). Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. PLoS ONE, 7(8).
Series/Report no.: PLoS ONE
Abstract: A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5′-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55×10−12 M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0042346
Schools: School of Physical and Mathematical Sciences 
Rights: © 2012 The Authors.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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