Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/95470
Title: Cyclic olefin copolymer based microfluidic devices for biochip applications : ultraviolet surface grafting using 2-methacryloyloxyethyl phosphorylcholine
Authors: Jena, Rajeeb Kumar
Yue, Chee Yoon
Keywords: DRNTU::Engineering::Mechanical engineering::Fluid mechanics
Issue Date: 2012
Source: Jena, R. K., & Yue, C. Y. (2012). Cyclic olefin copolymer based microfluidic devices for biochip applications: Ultraviolet surface grafting using 2-methacryloyloxyethyl phosphorylcholine. Biomicrofluidics, 6(1), 012822.
Series/Report no.: Biomicrofluidics
Abstract: This report studies the surface modification of cyclic olefin copolymer (COC) by 2-methacryloyloxyethyl phosphorylcholine (MPC) monomer using photografting technique for the purpose of biointerface applications, which demonstrate resistance to both protein adsorption and cell adhesion in COC-based microfluidic devices. This is essential because the hydrophobic nature of COC can lead to adsorption of specific compounds from biological fluids in the microchannel, which can affect the results during fluidic analysis and cause clogging inside the microchannel. A correlation was found between the irradiation time and hydrophobicity of the modified substrate. Static water contact angle results show that the hydrophilicity property of the MPC-grafted substrate improves with increasing irradiation time. The contact angle of the modified surface decreased to 20 ± 5° from 88 ± 3° for the untreated substrate. The surface characterization of the modified surface was evaluated using x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR spectroscopy). Attenuated total reflection-FTIR and XPS results show the presence of the phosphate group (P-O) on modified COC substrates, indicating that the hydrophilic MPC monomer has successfully grafted on COC. Finally, it was demonstrated that cell adhesion and protein adsorption on the MPC modified COC specimen has reduced significantly.
URI: https://hdl.handle.net/10356/95470
http://hdl.handle.net/10220/9295
ISSN: 1932-1058
DOI: 10.1063/1.3682098
Rights: © 2012 American Institute of Physics. This paper was published in Biomicrofluidics and is made available as an electronic reprint (preprint) with permission of American Institute of Physics. The paper can be found at the following official DOI: [http://dx.doi.org/10.1063/1.3682098]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

Files in This Item:
File Description SizeFormat 
2. Cyclic olefin copolymer based microfluidic.pdf1.9 MBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 10

50
Updated on Jan 31, 2023

Web of ScienceTM
Citations 10

44
Updated on Feb 6, 2023

Page view(s) 20

569
Updated on Feb 6, 2023

Download(s) 10

394
Updated on Feb 6, 2023

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.