Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/95674
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dc.contributor.authorWang, Leien
dc.contributor.authorShi, Yuen
dc.contributor.authorJu, Peijunen
dc.contributor.authorLiu, Ruien
dc.contributor.authorYeo, Siok Pingen
dc.contributor.authorXia, Yinyanen
dc.contributor.authorOwlanj, Hameden
dc.contributor.authorFeng, Zhiweien
dc.date.accessioned2013-03-05T07:30:59Zen
dc.date.accessioned2019-12-06T19:19:36Z-
dc.date.available2013-03-05T07:30:59Zen
dc.date.available2019-12-06T19:19:36Z-
dc.date.copyright2012en
dc.date.issued2012en
dc.identifier.citationWang, L., Shi, Y., Ju, P., Liu, R., Yeo, S. P., Xia, Y., et al. (2012). Silencing of Diphthamide Synthesis 3 (Dph3) Reduces Metastasis of Murine Melanoma. PLoS ONE, 7(11).en
dc.identifier.issn1932-6203en
dc.identifier.urihttps://hdl.handle.net/10356/95674-
dc.description.abstractMelanoma is the most dangerous skin cancer due to its highly metastatic potential and resistance to chemotherapy. Currently, there is no effective treatment for melanoma once it is progressed to metastatic stage. Therefore, further study to elucidate the molecular mechanism underlying the metastasis of melanoma cells is urgently required for the improvement of melanoma treatment. In the present study, we found that diphthamide synthesis 3 (Dph3) is involved in the metastasis of B16F10 murine melanoma cells by insertional mutagenesis. We demonstrated that Dph3 disruption impairs the migration of B16F10 murine melanoma cells. The requirement of Dph3 in the migration of melanoma cells was further confirmed by gene silencing with siRNA in vitro. In corresponding to this result, overexpression of Dph3 significantly promoted the migratory ability of B16F10 and B16F0 melanoma cells. Moreover, down regulation of Dph3 expression in B16F10 melanoma cells strikingly inhibits their cellular invasion and metastasis in vivo. Finally, we found that Dph3 promotes melanoma migration and invasion through the AKT signaling pathway. To conclude, our findings suggest a novel mechanism underlying the metastasis of melanoma cells which might serve as a new intervention target for the treatment of melanoma.en
dc.language.isoenen
dc.relation.ispartofseriesPLoS ONEen
dc.rights© 2012 The Authors.en
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleSilencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanomaen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1371/journal.pone.0049988en
dc.description.versionPublished versionen
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