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Title: Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes
Authors: Loo, Adeline Huiling
Bonanni, Alessandra
Ambrosi, Adriano
Poh, Hwee Ling
Pumera, Martin
Keywords: DRNTU::Engineering::Materials::Nanostructured materials
Issue Date: 2012
Source: Loo, A. H., Bonanni, A., Ambrosi, A., Poh, H. L., & Pumera, M. (2012). Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes. Nanoscale, 4(3), 921-925.
Series/Report no.: Nanoscale
Abstract: Immunosensors which display high sensitivity and selectivity are of utmost importance to the biomedical field. Graphene is a material which has immense potential for the fabrication of immunosensors. For the first time, we evaluate the immunosensing capabilities of various graphene surfaces in this work. We propose a simple and label-free electrochemical impedimetric immunosensor for immunoglobulin G (IgG) based on chemically modified graphene (CMG) surfaces such as graphite oxide, graphene oxide, thermally reduced graphene oxide and electrochemically reduced graphene oxide. Disposable electrochemical printed electrodes were first modified with CMG materials before anti-immunoglobulin G (anti-IgG), which is specific to IgG, was immobilized. The principle of detection lies in the changes in impedance spectra of the redox probe after the attachment of IgG to the immobilized anti-IgG. It was found that thermally reduced graphene oxide has the best performance when compared to the other CMG materials. In addition, the optimal concentration of anti-IgG to be deposited onto the modified electrode surface is 10 μg ml−1 and the linear range of detection of the immunosensor is from 0.3 μg ml−1 to 7 μg ml−1. Finally, the fabricated immunosensor also displays selectivity for IgG.
DOI: 10.1039/C2NR11492E
Schools: School of Physical and Mathematical Sciences 
Rights: © 2012 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Nanoscale, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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