Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96137
Title: Green preparation of antibiotic nanoparticle complex as potential anti-biofilm therapeutics via self-assembly amphiphile–polyelectrolyte complexation with dextran sulfate
Authors: Cheow, Wean Sin
Hadinoto, Kunn
Keywords: DRNTU::Science::Medicine::Biomedical engineering
Issue Date: 2011
Source: Cheow, W. S., & Hadinoto, K. (2012). Green preparation of antibiotic nanoparticle complex as potential anti-biofilm therapeutics via self-assembly amphiphile–polyelectrolyte complexation with dextran sulfate. Colloids and Surfaces B: Biointerfaces, 92, 55-63.
Series/Report no.: Colloids and surfaces B: biointerfaces
Abstract: Nanoscale antibiotic delivery has emerged as a promising therapeutic means to treat lung biofilm infection owed to its sputum penetrating ability. Due to the high antibiotic dosage requirement in anti-biofilm therapy, the most suitable formulation for this purpose is the antibiotic nanoparticles themselves, instead of the more extensively studied antibiotic-loaded nano-carriers, which often exhibit low drug loading. The present work details the preparation and characterization of antibiotic nanoparticle complex (or nanoplex) by self-assembly amphiphile–polyelectrolyte complexation process. Ofloxacin (OFX) and levofloxacin (LEV) are used as the antibiotics with dextran sulfate (DXT) as the polyelectrolyte. The nanoplex possesses high drug loading (up to 80%) and size < 400 nm ideal for sputum penetration. Unlike existing methods to prepare drug nanoparticles, the present method is fast, energy-minimal, solvent-free, and highly efficient as manifested in nearly 100% of drug is transformed into nanoplex. The effects of drug-to-polyelectrolyte charge ratio, pH, drug, and salt concentrations on the nanoplex characteristics (i.e. size, stability, drug loading) are investigated from which the optimal preparation conditions have been identified. Higher complexation efficiency and stronger agglomeration tendency are observed for LEV nanoplex owed to its higher hydrophobicity. The antibiotics are completely released from the nanoplex in aqueous salt solution within 3 h and their antimicrobial activity is preserved upon complexation. The nanoplex is readily transformed into amorphous dry powders that remain stable after one-month storage owed to the high glass transition temperature. The antibiotic nanoplexes are highly charged enabling their subsequent functionalization for targeted delivery and controlled drug release purposes.
URI: https://hdl.handle.net/10356/96137
http://hdl.handle.net/10220/10817
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2011.11.024
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2011 Elsevier B.V.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

SCOPUSTM   
Citations 10

41
Updated on Mar 22, 2024

Web of ScienceTM
Citations 10

38
Updated on Oct 24, 2023

Page view(s) 10

780
Updated on Mar 28, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.