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dc.contributor.authorMishra, Biswajiten
dc.contributor.authorBasu, Anindyaen
dc.contributor.authorChua, Ray Rong Yuanen
dc.contributor.authorSaravanan, Rathien
dc.contributor.authorTambyah, Paul Anantharajahen
dc.contributor.authorHo, Bowen
dc.contributor.authorChang, Matthew Wooken
dc.contributor.authorLeong, Susanna Su Janen
dc.identifier.citationMishra, B., Basu, A., Chua, R. R. Y., Saravanan, R., Tambyah, P. A., Ho, B., Chang, M. W., & Leong, S. S. J. (2014). Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens. Journal of Materials Chemistry B, 2(12),1706-1716.en
dc.description.abstractBacterial colonization of urinary catheters is a common problem leading to Catheter Associated Urinary Tract Infections (CAUTIs) in patients, which result in high treatment costs and associated complications. Due to the advantages of antimicrobial peptides (AMPs) compared to most other antimicrobial molecules, an increasing number of AMP-coated surfaces is being developed but their efficacy is hindered by suboptimal coating methods and loss of peptide activity upon surface tethering. This study aims to address this issue by employing a methodic approach that combines a simple selective chemical immobilization platform developed on a silicone catheter with the choice of a potent AMP, Lasioglossin-III (Lasio-III), to allow site specific immobilization of Lasio-III at an effective surface concentration. The Lasio-III peptide was chemically modified at the N-terminal with a cysteine residue to facilitate cysteine-directed immobilization of the peptide onto a commercial silicone catheter surface via a combination of an allyl glycidyl ether (AGE) brush and polyethylene glycol (PEG) based chemical coupling. The amount of immobilized peptide was determined to be 6.59 ± 0.89 μg cm−2 by Sulfo-SDTB assay. The AMP-coated catheter showed good antimicrobial activity against both Gram positive and negative bacteria. The antimicrobial properties of the AMP-coated catheter were sustained for at least 4 days post-incubation in a physiologically relevant environment and artificial urine and prevented the biofilm growth of E. coli and E. faecalis. Adenosine tri-phosphate leakage and propidium iodide fluorescence studies further confirmed the membranolytic mode of action of the immobilized peptide. To the best of our knowledge, this is the first proof-of-concept study that reports the efficacy of AMP immobilization by sulfhydryl coupling on a real catheter surfaceen
dc.format.extent12 p.en
dc.relation.ispartofseriesJournal of materials chemistry Ben
dc.rights© 2014 The Royal Society of Chemistry. This Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licenceen
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Bacteriaen
dc.titleSite specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogensen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolSchool of Materials Science & Engineeringen
dc.description.versionPublished versionen
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