Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96283
Title: Surface modification of polycaprolactone substrates using collagen-conjugated poly(methacrylic acid) brushes for the regulation of cell proliferation and endothelialisation
Authors: Yuan, Shaojun
Xiong, Gordon M.
Wang, Xiaoyan
Zhang, Sam
Choong, Cleo Swee Neo
Keywords: DRNTU::Engineering::Materials
Issue Date: 2012
Source: Yuan, S., Xiong, G. M., Wang, X., Zhang, S., & Choong, C. S. N. (2012). Surface modification of polycaprolactone substrates using collagen-conjugated poly(methacrylic acid) brushes for the regulation of cell proliferation and endothelialisation. Journal of Materials Chemistry, 22(26), 13039-13049.
Series/Report no.: Journal of materials chemistry
Abstract: The incorporation and presentation of cell recognition ligands on the surfaces of biodegradable blood-vessel implants to promote endothelialisation is considered to be a promising approach to prevent platelet aggregation and hence thrombogenesis. In this study, cell-adhesive collagen was covalently immobilised onto polycaprolactone (PCL) substrates via surface-initiated atom transfer radical polymerization (ATRP) to improve cell–material interactions. Functional polymer brushes of poly(methacrylic acid) (P(MAA)) containing dense and reactive carboxyl groups (–COOH) were formed on the PCL substrates in a controllable manner. The amount of collagen, which was conjugated to the pendant carboxyl groups via carbodiimide chemistry, increased with the concentration of –COOH groups on the grafted P(MAA) brushes. The affinity and growth of endothelial cells (ECs) were found to be significantly improved on the collagen-immobilised PCL substrates, and this improvement is positively correlated with the amount of covalently conjugated collagen. Thus, surface-initiated ATRP provides an alternative methodology for the surface functionalisation of biodegradable polyester scaffolds to enable the formation of a confluent layer of ECs. An optimally endothelialised material surface will play a major role in the minimisation of thrombogenicity and inflammation, and hence can be potentially used for vascular graft applications.
URI: https://hdl.handle.net/10356/96283
http://hdl.handle.net/10220/11326
DOI: 10.1039/c2jm31213a
Rights: © 2012 The Royal Society of Chemistry.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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