Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96324
Title: Improving signal-to-noise ratio of structured light microscopy based on photon reassignment
Authors: Singh, Vijay Raj
Choi, Heejin
Yew, Elijah Y. S.
Bhattacharya, Dipanjan
Yuan, Luo
Sheppard, Colin J. R.
Rajapakse, Jagath C.
Barbastathis, George
So, Peter T. C.
Issue Date: 2011
Source: Singh, V. R., Choi, H., Yew, E. Y. S., Bhattacharya, D., Yuan, L., Sheppard, C. J. R., et al. (2012). Improving signal-to-noise ratio of structured light microscopy based on photon reassignment. Biomedical Optics Express, 3(1), 206-214.
Series/Report no.: Biomedical optics express
Abstract: In this paper, we report a method for 3D visualization of a biological specimen utilizing a structured light wide-field microscopic imaging system. This method improves on existing structured light imaging modalities by reassigning fluorescence photons generated from off-focal plane excitation, improving in-focus signal strength. Utilizing a maximum likelihood approach, we identify the most likely fluorophore distribution in 3D that will produce the observed image stacks under structured and uniform illumination using an iterative maximization algorithm. Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods.
URI: https://hdl.handle.net/10356/96324
http://hdl.handle.net/10220/10165
DOI: 10.1364/BOE.3.000206
Rights: © 2011 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint (preprint) with permission of Optical Society of America. The paper can be found at the following official DOI: [http://dx.doi.org/10.1364/BOE.3.000206].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCSE Journal Articles

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