Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96401
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dc.contributor.authorAmali, Aseervatham Anushaen
dc.contributor.authorSie, Lawrenceen
dc.contributor.authorWinkler, Christophen
dc.contributor.authorFeatherstone, Marken
dc.date.accessioned2013-05-03T08:01:29Zen
dc.date.accessioned2019-12-06T19:30:03Z-
dc.date.available2013-05-03T08:01:29Zen
dc.date.available2019-12-06T19:30:03Z-
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationAmali, A. A., Sie, L., Winkler, C., & Featherstone, M. (2013). Zebrafish hoxd4a Acts Upstream of meis1.1 to Direct Vasculogenesis, Angiogenesis and Hematopoiesis. PLoS ONE, 8(3).en
dc.identifier.issn1932-6203en
dc.identifier.urihttps://hdl.handle.net/10356/96401-
dc.description.abstractMice lacking the 4th-group paralog Hoxd4 display malformations of the anterior vertebral column, but are viable and fertile. Here, we report that zebrafish embryos having decreased function of the orthologous hoxd4a gene manifest striking perturbations in vasculogenesis, angiogenesis and primitive and definitive hematopoiesis. These defects are preceded by reduced expression of the hemangioblast markers scl1, lmo2 and fli1 within the posterior lateral plate mesoderm (PLM) at 13 hours post fertilization (hpf). Epistasis analysis revealed that hoxd4a acts upstream of meis1.1 but downstream of cdx4 as early as the shield stage in ventral-most mesoderm fated to give rise to hemangioblasts, leading us to propose that loss of hoxd4a function disrupts hemangioblast specification. These findings place hoxd4a high in a genetic hierarchy directing hemangioblast formation downstream of cdx1/cdx4 and upstream of meis1.1. An additional consequence of impaired hoxd4a and meis1.1 expression is the deregulation of multiple Hox genes implicated in vasculogenesis and hematopoiesis which may further contribute to the defects described here. Our results add to evidence implicating key roles for Hox genes in their initial phase of expression early in gastrulation.en
dc.language.isoenen
dc.relation.ispartofseriesPLoS ONEen
dc.rights© 2013 The Author(s).en
dc.subjectDRNTU::Science::Biological sciences::Zoology::Morphologyen
dc.titleZebrafish hoxd4a acts upstream of meis1.1 to direct vasculogenesis, angiogenesis and hematopoiesisen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1371/journal.pone.0058857en
dc.description.versionPublished versionen
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