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dc.contributor.authorBao, Hongqianen
dc.contributor.authorPing, Yuanen
dc.contributor.authorPan, Yongzhengen
dc.contributor.authorLi, Linen
dc.contributor.authorLi, Junen
dc.contributor.authorGan, Leong Huaten
dc.identifier.citationBao, H., Ping, Y., Pan, Y., Li, L., Li, J., & Gan, L. H. (2012). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter, 8(8), 2518-2526.en
dc.description.abstractSerial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5–35.1 °C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of “gold-standard” PEI (25 kDa).en
dc.relation.ispartofseriesSoft matteren
dc.rights© 2012 The Royal Society of Chemistry.en
dc.titleThermo-responsive transfection of DNA complexes with well-defined chitosan terpolymersen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen
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