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Title: Design of preparative-supercritical fluid chromatography
Authors: Rajendran, Arvind
Keywords: DRNTU::Engineering::Chemical engineering
Issue Date: 2012
Source: Rajendran, A. (2012). Design of preparative-supercritical fluid chromatography. Journal of Chromatography A, 1250, 227-249.
Series/Report no.: Journal of chromatography A
Abstract: Preparative supercritical fluid chromatography (prep-SFC) is an important separation process in the chromatographers toolbox. Owing to the unique properties of the mobile phase, which is predominantly CO2, the behavior of SFC is markedly different from high performance liquid chromatography (HPLC). This review article focuses on the scale-up of preparative chromatography. The basics of SFC, with particular focus on highlighting the key differences between SFC and HPLC, are introduced. Then, a framework for rational design of prep-SFC is proposed. This framework is based on obtaining basic system parameters from analytical scale equipment, i.e., with very small amount of material, and performing design and optimization in silico to evaluate process performance and to identify operating conditions for scale-up. The tools required to obtain the input parameters such as adsorption isotherms are discussed and the development of the design and optimization framework is elaborated. Examples from the literature which use this approach for successful scale-up are provided. Finally the design of multi-column SFC systems is discussed.
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2012.05.037
Rights: © 2012 Elsevier B.V.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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