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dc.contributor.authorGupta, Archna P.en
dc.contributor.authorCrowley, Valerie M.en
dc.contributor.authorRovira-Graells, Núriaen
dc.contributor.authorPlanet, Evaristen
dc.contributor.authorMok, Sachelen
dc.contributor.authorPouplana, Llús Ribas deen
dc.contributor.authorPreiser, Peter Raineren
dc.contributor.authorBozdech, Zbyneken
dc.contributor.authorCortés, Alfreden
dc.identifier.citationRovira-Graells, N., Gupta, A. P., Planet, E., Crowley, V. M., Mok, S., Pouplana, L. R. d., et al. (2012). Transcriptional variation in the malaria parasite Plasmodium falciparum. Genome Research, 22(5), 925-938.en
dc.description.abstractMalaria genetic variation has been extensively characterized, but the level of epigenetic plasticity remains largely unexplored. Here we provide a comprehensive characterization of transcriptional variation in the most lethal malaria parasite, Plasmodium falciparum, based on highly accurate transcriptional analysis of isogenic parasite lines grown under homogeneous conditions. This analysis revealed extensive transcriptional heterogeneity within genetically homogeneous clonal parasite populations. We show that clonally variant expression controlled at the epigenetic level is an intrinsic property of specific genes and gene families, the majority of which participate in host–parasite interactions. Intrinsic transcriptional variability is not restricted to genes involved in immune evasion, but also affects genes linked to lipid metabolism, protein folding, erythrocyte remodeling, or transcriptional regulation, among others, indicating that epigenetic variation results in both antigenic and functional variation. We observed a general association between heterochromatin marks and clonally variant expression, extending previous observations for specific genes to essentially all variantly expressed gene families. These results suggest that phenotypic variation of functionally unrelated P. falciparum gene families is mediated by a common mechanism based on reversible formation of H3K9me3-based heterochromatin. In changing environments, diversity confers fitness to a population. Our results support the idea that P. falciparum uses a bet-hedging strategy, as an alternative to directed transcriptional responses, to adapt to common fluctuations in its environment. Consistent with this idea, we found that transcriptionally different isogenic parasite lines markedly differed in their survival to heat-shock mimicking febrile episodes and adapted to periodic heat-shock with a pattern consistent with natural selection of pre-existing parasites.en
dc.relation.ispartofseriesGenome researchen
dc.rights© 2012 Cold Spring Harbor Laboratory Press. This paper was published in Genome research and is made available as an electronic reprint (preprint) with permission of Cold Spring Harbor Laboratory Press. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleTranscriptional variation in the malaria parasite Plasmodium falciparumen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionPublished Versionen
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