Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/97411
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMishra, Biswajiten
dc.contributor.authorBasu, Anindyaen
dc.contributor.authorSaravanan, Rathien
dc.contributor.authorXiang, Lien
dc.contributor.authorYang, Lim Kaien
dc.contributor.authorLeong, Susanna Su Janen
dc.date.accessioned2013-06-04T08:19:32Zen
dc.date.accessioned2019-12-06T19:42:23Z-
dc.date.available2013-06-04T08:19:32Zen
dc.date.available2019-12-06T19:42:23Z-
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationMishra, B., Basu, A., Saravanan, R., Xiang, L., Yang, L. K., & Leong, S. S. J. (2013). Lasioglossin-III: antimicrobial characterization and feasibility study for immobilization applications. RSC Advances, 3(24), 9534-9543.en
dc.identifier.urihttps://hdl.handle.net/10356/97411-
dc.description.abstractPrevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser likelihood to incur pathogen resistance. This study presents a systematic approach towards evaluating the feasibility of Lasioglossin-III (Lasio-III) (a new bee venom AMP, found in Lasioglossum laticeps) to be tethered onto biodevices. Antimicrobial characterization of Lasio-III in solution confirms the peptide’s membranolytic mode of action and its salt-resistant, broad antimicrobial spectrum activity and anti-biofilm properties against Gram negative and Gram positive bacteria. Lassio-III was covalently immobilized on silicon surfaces using APTES and PEG spacers of varying lengths. Surface characterization of the AMP-immobilized silicon was done using water contact angle measurements, XPS analysis and ellipsometry. Even at modest surface peptide concentrations of y180 ng cm22 , Lassio-III showed antibacterial activities which were further enhanced with increasing PEG spacer lengths, as determined by live CFU counting and ATP leakage experiments. This proof-of-concept study demonstrates the potential of Lasio-III as an antimicrobial coating candidate.en
dc.language.isoenen
dc.relation.ispartofseriesRSC advancesen
dc.rights© 2013 Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by RSC advances, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1039/C3RA40887F].en
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Bacteriaen
dc.titleLasioglossin-III : antimicrobial characterization and feasibility study for immobilization applicationsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1039/C3RA40887Fen
dc.description.versionAccepted versionen
dc.identifier.rims171651en
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:MSE Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.