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Title: Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications
Authors: Mishra, Biswajit
Basu, Anindya
Saravanan, Rathi
Xiang, Li
Yang, Lim Kai
Leong, Susanna Su Jan
Keywords: DRNTU::Science::Biological sciences::Microbiology::Bacteria
Issue Date: 2013
Source: Mishra, B., Basu, A., Saravanan, R., Xiang, L., Yang, L. K., & Leong, S. S. J. (2013). Lasioglossin-III: antimicrobial characterization and feasibility study for immobilization applications. RSC Advances, 3(24), 9534-9543.
Series/Report no.: RSC advances
Abstract: Prevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser likelihood to incur pathogen resistance. This study presents a systematic approach towards evaluating the feasibility of Lasioglossin-III (Lasio-III) (a new bee venom AMP, found in Lasioglossum laticeps) to be tethered onto biodevices. Antimicrobial characterization of Lasio-III in solution confirms the peptide’s membranolytic mode of action and its salt-resistant, broad antimicrobial spectrum activity and anti-biofilm properties against Gram negative and Gram positive bacteria. Lassio-III was covalently immobilized on silicon surfaces using APTES and PEG spacers of varying lengths. Surface characterization of the AMP-immobilized silicon was done using water contact angle measurements, XPS analysis and ellipsometry. Even at modest surface peptide concentrations of y180 ng cm22 , Lassio-III showed antibacterial activities which were further enhanced with increasing PEG spacer lengths, as determined by live CFU counting and ATP leakage experiments. This proof-of-concept study demonstrates the potential of Lasio-III as an antimicrobial coating candidate.
DOI: 10.1039/C3RA40887F
Rights: © 2013 Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by RSC advances, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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