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Title: Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity
Authors: Luo, Rongcong
Shaillender, Mutukumaraswamy
Venkatraman, Subbu S.
Neu, Björn
Keywords: DRNTU::Engineering::Materials::Biomaterials
Issue Date: 2012
Source: Luo, R., Shaillender, M., Venkatraman, S. S., & Neu, B. (2012). Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity. Journal of materials science : materials in medicine, 23(1), 63-71.
Series/Report no.: Journal of materials science : Materials in medicine
Abstract: This work reports the fabrication of layer-by-layer (LbL) polyelectrolyte coated erythrocyte carriers that provide a simple means for controlling the burst and subsequent release of lysozyme. Erythrocytes were loaded with RITC-lysozyme as model compound via the hypotonic dialysis method. An encapsulation efficiency of 41.6% and a loading amount of 12.7 pg/cell was achieved. It is demonstrated that these carriers maintain their shape and integrity similar to natural erythrocytes after the encapsulation procedures, and achieve a uniform distribution of the encapsulated lysozyme. The erythrocyte carriers were fixed with glutaraldehyde and then successfully coated with biocompatible polyelectrolytes, poly-l-lysine hydrobromide and dextran sulfate, using the LbL method. It is demonstrated that the release profile of the encapsulated macromolecule can be regulated by adjusting the number of polyelectrolyte layers. Furthermore by adjusting the concentrations of the cross linking agent the activity of the encapsulated lysozyme can be well preserved. These core–shell microcapsules, consisting of erythrocytes loaded with bioactive substances and coated with a polyelectrolyte multilayer shell, hold promise for a new type of biocompatible and biodegradable drug delivery system.
DOI: 10.1007/s10856-011-4485-2
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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