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Title: Study of immobilization procedure on silver nanolayers and detection of estrone with diverged beam Surface Plasmon Resonance (SPR) Imaging
Authors: Karabchevsky, Alina
Tsapovsky, Lev
Marks, Robert
Abdulhalim, Ibrahim
Issue Date: 2013
Source: Karabchevsky, A., Tsapovsky, L., Marks, R.,& Abdulhalim, I. (2013). Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging. Biosensors, 3(1), 157-170.
Series/Report no.: Biosensors
Abstract: An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor—estrone—was attached and detected in deionized water with a diverging beam SPR imaging sensor.
ISSN: 2079-6374
DOI: 10.3390/bios3010157
Rights: © 2013 MDPI. This paper was published in Biosensors and is made available as an electronic reprint (preprint) with permission of MDPI. The paper can be found at the following official DOI: [].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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