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Title: Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus
Authors: Cheng, Ming Soon
Ho, Jia Shin
Tan, Cheong Huat
Wong, Jeslyn Pei Sze
Ng, Lee Ching
Toh, Chee-Seng
Issue Date: 2012
Source: Cheng, M. S., Ho, J. S., Tan, C. H., Wong, J. P. S., Ng, L. C., & Toh, C.-S. (2012). Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Analytica Chimica Acta, 725, 74-80.
Series/Report no.: Analytica chimica acta
Abstract: A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) and DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL−1 with linear range from 1 to 103 pfu mL−1 (R2 = 0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus and dengue type 3 virus (DENV-3). Relative standard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor.
ISSN: 0003-2670
DOI: 10.1016/j.aca.2012.03.017
Rights: © 2012 Elsevier B.V.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SPMS Journal Articles

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