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Title: Advanced immobilization and amplification for high performance protein chips
Authors: Liu, Yingshuai
Li, Chang Ming
Issue Date: 2012
Source: Liu, Y., & Li, C. M. (2012). Advanced Immobilization and Amplification for High Performance Protein Chips. Analytical Letters, 45(2-3), 130-155.
Series/Report no.: Analytical letters
Abstract: With the success of high-throughput DNA microarrays, protein biochips have been intensively investigated and broadly used in bioscience research, clinic diagnosis, drug discovery, and other applications. However, there is great need to significantly improve the sensitivity of protein chips, especially in early diagnosis. A major challenge of improving sensitivity is that protein detection does not have an effective amplification method, such as PCR for DNA microarrays. Construction of unique biofilms for efficient immobilization of protein probes and innovation of new amplification schemes could play a critical role in performance improvement of protein biochips. With dramatic developments in microfabrication, nanotechnologies, and biotechnologies, enormous progress has been made, particularly in improving biosensing sensitivity. This article reviews new advances in protein biochip technologies with emphasis on novel approaches for efficient probe immobilization and nanomaterials-assisted signal amplification for high performance protein chips. Prominent progress in integration of protein microarrays with microfluidic platforms is briefly discussed. The major challenges and perspectives on the future of protein biochips are also addressed.
DOI: 10.1080/00032719.2011.633187
Rights: © 2012 Taylor & Francis Group, LLC.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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