Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/98614
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWang, Huijuanen
dc.contributor.authorListrat, Anneen
dc.contributor.authorMeunier, Brunoen
dc.contributor.authorGueugneau, Marineen
dc.contributor.authorCoudy-Gandilhon, Cécileen
dc.contributor.authorCombaret, Lydieen
dc.contributor.authorTaillandier, Danielen
dc.contributor.authorPolge, Cécileen
dc.contributor.authorAttaix, Didieren
dc.contributor.authorLethias, Claireen
dc.contributor.authorLee, Kijoonen
dc.contributor.authorGoh, Kheng Limen
dc.contributor.authorBéchet, Danielen
dc.date.accessioned2014-01-10T01:44:01Zen
dc.date.accessioned2019-12-06T19:57:38Z-
dc.date.available2014-01-10T01:44:01Zen
dc.date.available2019-12-06T19:57:38Z-
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationWang, H., Listrat, A., Meunier, B., Gueugneau, M., Coudy-Gandilhon, C., Combaret, L., et al. (2013). Apoptosis in capillary endothelial cells in ageing skeletal muscle. Aging cell, 1-9.en
dc.identifier.issn1474-9718en
dc.identifier.urihttps://hdl.handle.net/10356/98614-
dc.description.abstractThe age-related loss of skeletal muscle mass and function (sarcopenia) is a consistent hallmark of ageing. Apoptosis plays an important role in muscle atrophy, and the intent of this study was to specify whether apoptosis is restricted to myofibre nuclei (myonuclei) or occurs in satellite cells or stromal cells of extracellular matrix (ECM). Sarcopenia in mouse gastrocnemius muscle was characterized by myofibre atrophy, oxidative type grouping, delocalization of myonuclei and ECM fibrosis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) indicated a sharp rise in apoptosis during ageing. TUNEL coupled with immunostaining for dystrophin, paired box protein-7 (Pax7) or laminin-2α, respectively, was used to identify apoptosis in myonuclei, satellite cells and stromal cells. In adult muscle, apoptosis was not detected in myofibres, but was restricted to stromal cells. Moreover, the age-related rise in apoptotic nuclei was essentially due to stromal cells. Myofibre-associated apoptosis nevertheless occurred in old muscle, but represented < 20% of the total muscle apoptosis. Specifically, apoptosis in old muscle affected a small proportion (0.8%) of the myonuclei, but a large part (46%) of the Pax7+ satellite cells. TUNEL coupled with CD31 immunostaining further attributed stromal apoptosis to capillary endothelial cells. Age-dependent rise in apoptotic capillary endothelial cells was concomitant with altered levels of key angiogenic regulators, perlecan and a perlecan domain V (endorepellin) proteolytic product. Collectively, our results indicate that sarcopenia is associated with apoptosis of satellite cells and impairment of capillary functions, which is likely to contribute to the decline in muscle mass and functionality during ageing.en
dc.language.isoenen
dc.relation.ispartofseriesAging cellen
dc.rights© 2013 The Authors. This paper was published in Aging Cell and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1111/acel.12169]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Engineering::Bioengineeringen
dc.titleApoptosis in capillary endothelial cells in ageing skeletal muscleen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1111/acel.12169en
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SCBE Journal Articles
Files in This Item:
File Description SizeFormat 
Apoptosis in capillary endothelial cells in ageing skeletal muscle.pdf497.99 kBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations

47
checked on Sep 4, 2020

WEB OF SCIENCETM
Citations

48
checked on Oct 23, 2020

Page view(s)

422
checked on Oct 24, 2020

Download(s)

215
checked on Oct 24, 2020

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.