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Title: Apoptosis in capillary endothelial cells in ageing skeletal muscle
Authors: Wang, Huijuan
Listrat, Anne
Meunier, Bruno
Gueugneau, Marine
Coudy-Gandilhon, Cécile
Combaret, Lydie
Taillandier, Daniel
Polge, Cécile
Attaix, Didier
Lethias, Claire
Lee, Kijoon
Goh, Kheng Lim
Béchet, Daniel
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2013
Source: Wang, H., Listrat, A., Meunier, B., Gueugneau, M., Coudy-Gandilhon, C., Combaret, L., et al. (2013). Apoptosis in capillary endothelial cells in ageing skeletal muscle. Aging cell, 1-9.
Series/Report no.: Aging cell
Abstract: The age-related loss of skeletal muscle mass and function (sarcopenia) is a consistent hallmark of ageing. Apoptosis plays an important role in muscle atrophy, and the intent of this study was to specify whether apoptosis is restricted to myofibre nuclei (myonuclei) or occurs in satellite cells or stromal cells of extracellular matrix (ECM). Sarcopenia in mouse gastrocnemius muscle was characterized by myofibre atrophy, oxidative type grouping, delocalization of myonuclei and ECM fibrosis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) indicated a sharp rise in apoptosis during ageing. TUNEL coupled with immunostaining for dystrophin, paired box protein-7 (Pax7) or laminin-2α, respectively, was used to identify apoptosis in myonuclei, satellite cells and stromal cells. In adult muscle, apoptosis was not detected in myofibres, but was restricted to stromal cells. Moreover, the age-related rise in apoptotic nuclei was essentially due to stromal cells. Myofibre-associated apoptosis nevertheless occurred in old muscle, but represented < 20% of the total muscle apoptosis. Specifically, apoptosis in old muscle affected a small proportion (0.8%) of the myonuclei, but a large part (46%) of the Pax7+ satellite cells. TUNEL coupled with CD31 immunostaining further attributed stromal apoptosis to capillary endothelial cells. Age-dependent rise in apoptotic capillary endothelial cells was concomitant with altered levels of key angiogenic regulators, perlecan and a perlecan domain V (endorepellin) proteolytic product. Collectively, our results indicate that sarcopenia is associated with apoptosis of satellite cells and impairment of capillary functions, which is likely to contribute to the decline in muscle mass and functionality during ageing.
ISSN: 1474-9718
DOI: 10.1111/acel.12169
Rights: © 2013 The Authors. This paper was published in Aging Cell and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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