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Title: Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery
Authors: Yan, Jiang
Korolev, Nikolay
Eom, Khee Dong
Nordenskiöld, Lars
Tam, James P.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2011
Source: Yan, J., Korolev, N., Eom, K. D., Tam, J. P.,& Nordenskiöld, L. (2012). Biophysical Properties and Supramolecular Structure of Self-Assembled Liposome/ε-Peptide/DNA Nanoparticles: Correlation with Gene Delivery. Biomacromolecules, 13(1), 124-131.
Series/Report no.: Biomacromolecules
Abstract: Using solid-phase synthesis, lysine can be oligomerized by a reaction of the peptide carboxylate with the ε-amino group to produce nontoxic, biodegradable cationic peptides, ε-oligo(l-lysines). Here α-substituted derivatives of such ε-oligo(l-lysines) containing arginine and histidine in the side chain were tested as vectors for in vitro gene delivery. Combination of ε-oligolysines with the cationic lipid DOTAP and plasmid DNA resulted in transfection efficiency exceeding that of DOTAP alone, without significant increase in cytotoxicity. Synchrotron small-angle X-ray scattering studies revealed self-assembly of the DOTAP, ε-oligolysines, and DNA to ordered lamellar complexes. High transfection efficiency of the nanoparticles correlates with increase in zeta potential above +20 mV and requires particle size to be below 500 nm. The synergistic effect of branched ε-oligolysines and DOTAP in gene delivery can be explained by the increase in surface charge and by the supramolecular structure of the DOTAP/ε-oligolysine/DNA nanoparticles.
DOI: 10.1021/bm201359r
Fulltext Permission: none
Fulltext Availability: No Fulltext
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