Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/99308
Full metadata record
DC FieldValueLanguage
dc.contributor.authorQian, Chenen
dc.contributor.authorWong, Chui Chingen
dc.contributor.authorSwarup, Sanjayen
dc.contributor.authorChiam, Keng-Hweeen
dc.date.accessioned2013-11-07T07:28:43Zen
dc.date.accessioned2019-12-06T20:05:37Z-
dc.date.available2013-11-07T07:28:43Zen
dc.date.available2019-12-06T20:05:37Z-
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationQian, C., Wong, C. C., Swarup, S., & Chiam, K. H. (2013). Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility. Applied and environmental microbiology, 79(15), 4734-4743.en
dc.identifier.issn0099-2240en
dc.identifier.urihttps://hdl.handle.net/10356/99308-
dc.description.abstractWe have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of the cell's rotational trajectories. The second applies piecewise linear approximation to the accumulated rotation curve to reduce noise and separate the motion of bacteria into phases. Thus, it can separate counterclockwise (CCW) and clockwise (CW) rotations distinctly and measure rotational speed accurately. Using this program, we analyzed the properties of tethered Pseudomonas aeruginosa and Pseudomonas putida cells for the first time. We found that the Pseudomonas flagellar motor spends equal time in both CCW and CW phases and that it rotates with the same speed in both phases. In addition, we discovered that the cell body can remain stationary for short periods of time, leading to the existence of a third phase of the flagellar motor which we call “pause.” In addition, P. aeruginosa cells adopt longer run lengths, fewer pause frequencies, and shorter pause durations as part of their chemotactic response. We propose that one purpose of the pause phase is to allow the cells to turn at a large angle, where we show that pause durations in free-swimming cells positively correlate with turn angle sizes. Taken together, our results suggest a new “run-reverse-turn” paradigm for polar-flagellated Pseudomonas motility that is different from the “run-and-tumble” paradigm established for peritrichous Escherichia coli.en
dc.language.isoenen
dc.relation.ispartofseriesApplied and environmental microbiologyen
dc.subjectDRNTU::Science::Biological sciences::Microbiologyen
dc.titleBacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motilityen
dc.typeJournal Articleen
dc.identifier.doi10.1128/AEM.01027-13en
dc.identifier.pmid23728820-
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:SCELSE Journal Articles

SCOPUSTM   
Citations 10

43
Updated on Dec 3, 2022

Web of ScienceTM
Citations 10

43
Updated on Nov 30, 2022

Page view(s) 10

732
Updated on Dec 6, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.