Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/99463
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dc.contributor.authorSong, Kedongen
dc.contributor.authorWang, Haien
dc.contributor.authorZhang, Bowenen
dc.contributor.authorLim, Mayasarien
dc.contributor.authorLiu, YingChaoen
dc.contributor.authorLiu, Tianqingen
dc.date.accessioned2013-08-02T03:29:28Zen
dc.date.accessioned2019-12-06T20:07:47Z-
dc.date.available2013-08-02T03:29:28Zen
dc.date.available2019-12-06T20:07:47Z-
dc.date.copyright2012en
dc.date.issued2012en
dc.identifier.citationSong, K., Wang, H., Zhang, B., Lim, M., Liu, Y.,& Liu, T. (2013). Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects. Cell Stress and Chaperones, 18(2), 193-201.en
dc.identifier.urihttps://hdl.handle.net/10356/99463-
dc.identifier.urihttp://hdl.handle.net/10220/12852en
dc.description.abstractIn this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects.en
dc.language.isoenen
dc.relation.ispartofseriesCell stress and chaperonesen
dc.titleNumerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defectsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doihttp://dx.doi.org/10.1007/s12192-012-0370-2en
item.grantfulltextnone-
item.fulltextNo Fulltext-
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