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Title: Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages
Authors: Nair, B.
Sykes, D. E.
Mammen, M. J.
Hui, R.
Schwartz, Stanley A.
Reynolds, Jessica L.
Prasad, Paras N.
Mahajan, Supriya D.
Law, Wing-Cheung
Aalinkeel, Ravikumar
Yong, Ken-Tye
Keywords: DRNTU::Engineering::Electrical and electronic engineering
Issue Date: 2012
Source: Reynolds, J. L., Law, W.-C., Mahajan, S. D., Aalinkeel, R., Nair, B., Sykes, D. E., Mammen, M. J., Yong, K. T., Hui, R., Prasad, P. N., & Schwartz, S. A. (2012). Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages. The journal of immunology, 188(8), 3757-3765.
Series/Report no.: The journal of immunology
Abstract: Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they play a role in the development of this disease, as well as impact the overall course of disease progression. Galectin-1 is a member of a family of β-galactoside-binding lectins that are soluble adhesion molecules and that mediate direct cell-pathogen interactions during HIV-1 viral adhesion. Because the drug abuse epidemic and the HIV-1 epidemic are closely interrelated, we propose that increased expression of galectin-1 induced by morphine may modulate HIV-1 infection of human monocyte-derived macrophages (MDMs). In this article, we show that galectin-1 gene and protein expression are potentiated by incubation with morphine. Confirming previous studies, morphine alone or galectin-1 alone enhance HIV-1 infection of MDMs. Concomitant incubation with exogenous galectin-1 and morphine potentiated HIV-1 infection of MDMs. We used a nanotechnology approach that uses gold nanorod-galectin-1 small interfering RNA complexes (nanoplexes) to inhibit gene expression for galectin-1. We found that nanoplexes silenced gene expression for galectin-1, and they reversed the effects of morphine on galectin-1 expression. Furthermore, the effects of morphine on HIV-1 infection were reduced in the presence of the nanoplex.
DOI: 10.4049/jimmunol.1102276
Fulltext Permission: none
Fulltext Availability: No Fulltext
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