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Preferential interaction of the mRNA proofreading factor TFIIS zinc ribbon with rU·dA base pairs correlates with its function
The transcriptional factor TFIIS helps overcome elongation barriers and enhances proofreading by RNA polymerase II. These TFIIS functions may be modulated by the TFIIS zinc ribbon domain through interactions with nucleic ...
Novel zinc finger motif in the basal transcriptional machinery : three-dimensional NMR studies of the nucleic acid binding domain of transcriptional elongation factor TFIIS
Transcriptional elongation provides a key control point in the regulation of eukaryotic gene expression. Here we describe homonuclear and lSN-heteronuclear 3D NMR studies of the nucleic acid binding domain ...
Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS
The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues ...
The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II
The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant ...
Structure of a new nucleic-acid-binding motif in eukaryotic transcriptional elongation factor TFIIS
Transcriptional elongation involves dynamic interactions among RNA polymerase and single-stranded and double stranded nucleic acids in the ternary complex1–4. In prokaryotes its regulation pro-vides an important mechanism ...
Cloning, expression and characterization of the human transcription elongation factor, TFIIS
The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coil with the T7 expression system. This 280-amino acid TFIIS protein is shorter by 21 residues than that of the mouse. The missing 21 ...