Now showing items 31-40 of 68
Expression, purification and characterization of C2 domain of milk fat globule-EGF-factor 8-L
Milk fat globule-EGF-factor 8-L (MFG-E8L) is secreted by activated macrophages and functions as a linker protein or opsonin between the dying cells and phagocytes. MFG-E8L recognizes the apoptotic or dying cells by ...
The flexible loop of Bcl-2 is required for molecular interaction with immunosuppressant FK-506 binding protein 38 (FKBP38)
Bcl-2 contains an unusually long loop between the first and the second helices. This loop has been shown to be highly flexible based on NMR and X-ray crystallographic analyses of this region. Bcl-2 is regulated at the ...
Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional ...
NMR and crystallographic structures of the FK506 binding domain of human malarial parasite Plasmodium vivax FKBP35
The emergence of drug-resistant malaria parasites is the major threat to effective malaria control, prompting a search for novel compounds with mechanisms of action that are different from the traditionally used drugs. The ...
Solution structure of FK506 binding domain (FKBD) of Plasmodium falciparum FK506 binding protein 35 (PfFKBP35)
No abstract available.
Co-delivery of drugs and DNA from cationic core–shell nanoparticles self-assembled from a biodegradable copolymer
Non-viral gene-delivery systems are safer to use and easier to produce than viral vectors, but their comparatively low transfection efficiency has limited their applications. Co-delivery of drugs and DNA has been proposed ...
Molecular mimicry-based repositioning of Nutlin-3 to anti-apoptotic Bcl-2 family proteins
The identification of off-target binding of drugs is a key to repositioning drugs to new therapeutic categories. Here we show the universal interactions of the p53 transactivation domain (p53TAD) with ...
Backbone 1H, 13C, and 15N resonance assignments of the N-terminal domain of FKBP38 (FKBP38NTD)
FK-506 binding protein 38 (FKBP38) interacts with Bcl-2/Bcl-Xl and helps them localize at the mitochondrial membrane (Shirane and Nakayama, 2003). The down-regulation of FKBP38 was shown to influence ...
The natively disordered loop of Bcl-2 undergoes phosphorylation-dependent conformational change and interacts with Pin1
Bcl-2 plays a central role in the regulation of apoptosis. Structural studies of Bcl-2 revealed the presence of a flexible and natively disordered loop that bridges the Bcl-2 homology motifs, BH3 and BH4. This loop is ...
X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death
THE Bcl-2 family of proteins regulate programmed cell death by an unknown mechanism1. Here we describe the crystal and solution structures of a Bcl-2 family member, Bcl-xL (ref. 2). The structures consist of two central, ...